# Daniel B. Stribling # Renne Lab, University of Florida # Hybkit Project : http://www.github.com/RenneLab/hybkit

Example Target Analysis

This directory contains a sample analysis of Hyb-format data, published in the quick Crosslinking and Sequencing of Hybrids (qCLASH) experiment described in: Gay, Lauren A., et al. “Modified cross-linking, ligation, and sequencing of hybrids (qCLASH) identifies Kaposi’s Sarcoma-associated herpesvirus microRNA targets in endothelial cells.” Journal of virology 92.8 (2018): e02138-17.

This analysis specifically investigates and characterizes miRNA arising from Kaposi’s Sarcoma Herpesvirus, which are given the type name “KSHV_miRNA”. Both individual and summary output files are produced.

Hybrid sequence information created by the Hyb program information is available at NCBI Gene Expression Ombnibus (GEO) GSE101978, at:

The data files can be downloaded and uncompressed by using the command:

$ sh ./download_data.sh

The unpacked hyb data-file require ~130 Mb of space. The completed output of the analysis requires ~20 Mb of space.

Target Analysis Example Output

_images/GSM2720020_WT_BR1_KSHV_only_kshv-miR-K12-5.png _images/GSM2720020_WT_BR1_KSHV_only_kshv-miR-K12-5_types.png