Example Summary Analysis

This directory contains a sample analysis of Hyb-format data, published in the quick Crosslinking and Sequencing of Hybrids (qCLASH) experiment described in: Gay, Lauren A., et al. “Modified cross-linking, ligation, and sequencing of hybrids (qCLASH) identifies Kaposi’s Sarcoma-associated herpesvirus microRNA targets in endothelial cells.” Journal of virology 92.8 (2018): e02138-17.

This analysis first performs quality control on the data. It then summarizes and analyzes the hybrid, segment, and miRNA characteristics of each input file. Analyses for each individual file, and a combined summary analysis are all produced.

The sequencing information is available at NCBI Gene Expression Ombnibus (GEO) GSE101978, at:

The data files can be downloaded and uncompressed by using the command:

$ sh ./download_data.sh

The unpacked hyb data-files require ~2 GB of space. The completed output of the analysis requires ~1.5 GB of space.

Summary Analysis Example Output

_images/combined_analysis_type_hybrids.png _images/combined_analysis_type_segall.png _images/combined_analysis_mirna_counts.png