Example Summary Analysis¶
This directory contains a sample analysis of Hyb-format data, published in the quick Crosslinking and Sequencing of Hybrids (qCLASH) experiment described in: Gay, Lauren A., et al. “Modified cross-linking, ligation, and sequencing of hybrids (qCLASH) identifies Kaposi’s Sarcoma-associated herpesvirus microRNA targets in endothelial cells.” Journal of virology 92.8 (2018): e02138-17.
This analysis first performs quality control on the data. It then summarizes and analyzes the hybrid, segment, and miRNA characteristics of each input file. Analyses for each individual file, and a combined summary analysis are all produced.
The sequencing information is available at NCBI Gene Expression Ombnibus (GEO) GSE101978, at:
The data files can be downloaded and uncompressed by using the command:
$ sh ./download_data.sh
The unpacked hyb data-files require ~2 GB of space. The completed output of the analysis requires ~1.5 GB of space.